The effect of gamma radiation processing on the lipid content, fatty acid composition and conjugated linoleic acid (CLA) profile in frozen lamb meat was investigated. Samples of longissimus thoracis muscle from lambs fed lucerne basal diets either unsupplemented or supplemented with polyunsaturated vegetable oils were irradiated (7kGy) and analysed. CLA contents in lamb meat did not affect (P>0.05) the levels of lipid oxidation induced by the irradiation. No significant differences (P>0.05) were observed for fatty acid composition, related nutritional indexes (n-6/n-3 and PUFA/SFA), as well as for total lipid and CLA contents, between non-irradiated (control) and irradiated meat samples. In contrast, meat irradiation affected the relative proportions of total trans,trans and cis/trans CLA isomers (P<0.001), in addition to the percentage of some minor individual CLA isomers (t11,t13 and t9,t11, with P<0.05 and P<0.001, respectively). The percentage of total cis/trans CLA isomers slightly decreased in irradiated samples, while the relative proportion of total trans,trans isomers slightly increased. This observation may be explained by the higher susceptibility to autoxidation of the cis double bond relative to the trans configuration.