High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type"

Pierre E Douarre; William Cashman; Jim Buckley; Aidan Coffey; Jim M O'Mahony

doi:10.1016/j.mimet.2011.10.009

High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type"

J Microbiol Methods. 2012 Jan;88(1):172-4. doi: 10.1016/j.mimet.2011.10.009. Epub 2011 Oct 18.

Authors

Pierre E Douarre¹, William Cashman, Jim Buckley, Aidan Coffey, Jim M O'Mahony

Affiliation

¹ Cork Institute of Technology, Cork, Ireland.

PMID: 22033498
DOI: 10.1016/j.mimet.2011.10.009

Abstract

In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.

Publication types

Evaluation Study

MeSH terms

Animals
Bacterial Typing Techniques / economics
Bacterial Typing Techniques / methods*
Cattle
Cattle Diseases / microbiology*
Genotype
Mycobacterium avium subsp. paratuberculosis / classification
Mycobacterium avium subsp. paratuberculosis / genetics
Mycobacterium avium subsp. paratuberculosis / isolation & purification*
Paratuberculosis / microbiology*
Polymerase Chain Reaction / economics
Polymerase Chain Reaction / methods*
Polymorphism, Genetic
Sheep
Sheep Diseases / microbiology*
Tandem Repeat Sequences