Abstract
To investigate the performance of a nucleic acid amplification test (NAAT) for the diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Bacteremia / diagnosis*
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Bacteremia / microbiology
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Bacteriological Techniques / methods*
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Blood / microbiology*
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Humans
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Middle Aged
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / isolation & purification*
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Real-Time Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Specimen Handling / methods*
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Tuberculosis / diagnosis*
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Tuberculosis / microbiology
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Young Adult