Abstract
Introduction:
Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE).
Methods:
The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program.
Results:
Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%.
Conclusions:
RT-PCR provides same day results, however it showed low sensitivity for VRE detection.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Carbon-Oxygen Ligases / genetics*
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Enterococcus / genetics*
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Enterococcus / isolation & purification
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Gram-Positive Bacterial Infections / diagnosis*
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Gram-Positive Bacterial Infections / microbiology
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Humans
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Predictive Value of Tests
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Real-Time Polymerase Chain Reaction / methods
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Rectum / microbiology*
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Sensitivity and Specificity
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Vancomycin Resistance / genetics*
Substances
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Bacterial Proteins
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VanA ligase, Bacteria
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VanB protein, Enterococcus
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Carbon-Oxygen Ligases