The service reorganization is a part of the healthcare system reform. Some hospitals may not be able to provide all services in a 24-h basis. This evaluation was on all night-time (22 : 00 p.m. to 07 : 00 a.m.) interfacility transport by Alice Ho Miu Ling Hospital emergency department from January 2008 to December 2010, which were in-patients from nonemergency wards. The safety, speed, and performance were analyzed. During the study period, 73 cases were transferred out. Majority of them were having neurosurgical emergency (n=21, 29%) or surgical emergency (n=34, 46%). En-route physiological deteriorations were encountered in 4% (3/73) of cases. The mean acceptance time was 8 min and the team mobilization time was 13 min on average. The total service time ranged from 40 to 115 min. In conclusion, en-route adverse event was not rare. The specialized team can act as a facilitator and coordinator to improve the safety and effectiveness of the whole process.