[In vitro strategies for human gametes production from stem cells]

Med Sci (Paris). 2011 Oct;27(10):866-74. doi: 10.1051/medsci/20112710015. Epub 2011 Oct 21.
[Article in French]

Abstract

Embryonic stem cells (ESC) are self-renewal and pluripotent cells that are able to differentiate in vitro into several cell types in favourable conditions. Technical protocols for in vitro gametes production have been developed in mice and human species. The functionality of such differentiated cells is not always analysed and an early meiotic arrest is a current observation. These kinds of experimentations have also been tested from human induced pluripotent stem cells (IPSC). However, differentiation ends shortly at the primordial germ cell stage.

Publication types

  • English Abstract
  • Review

MeSH terms

  • Animals
  • Cell Lineage
  • Cells, Cultured / cytology
  • Cells, Cultured / drug effects
  • Cellular Microenvironment
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / drug effects
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Transfer Techniques
  • Hormones / pharmacology
  • Humans
  • In Vitro Techniques
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / drug effects
  • Intercellular Signaling Peptides and Proteins / pharmacology
  • Male
  • Mice
  • Oocytes / cytology*
  • Oogenesis / drug effects*
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / drug effects
  • Sperm Injections, Intracytoplasmic
  • Spermatocytes / cytology*
  • Spermatogenesis / drug effects*

Substances

  • Hormones
  • Intercellular Signaling Peptides and Proteins