Display of the antigenic region of Nipah virus nucleocapsid protein on hepatitis B virus capsid

Wei Boon Yap; Beng Ti Tey; Noorjahan Banu Mohamed Alitheen; Wen Siang Tan

doi:10.1016/j.jbiosc.2011.09.007

Display of the antigenic region of Nipah virus nucleocapsid protein on hepatitis B virus capsid

J Biosci Bioeng. 2012 Jan;113(1):26-9. doi: 10.1016/j.jbiosc.2011.09.007. Epub 2011 Oct 24.

Authors

Wei Boon Yap¹, Beng Ti Tey, Noorjahan Banu Mohamed Alitheen, Wen Siang Tan

Affiliation

¹ Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

PMID: 22024533
DOI: 10.1016/j.jbiosc.2011.09.007

Abstract

The C-terminal domain of Nipah virus (NiV) nucleocapsid protein (NP₄₀₁₋₅₃₂) was inserted at the N-terminus and the immunodominant loop of hepatitis B core antigen (HBc). The stability of NP₄₀₁₋₅₃₂ increased tremendously when displayed on the HBc particles. These particles reacted specifically with the swine anti-NiV and the human anti-HBc antisera.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Viral / immunology*
Capsid / metabolism*
Epitopes / immunology
Hepatitis B Antibodies / immunology
Hepatitis B Core Antigens / immunology
Hepatitis B virus / metabolism*
Humans
Nipah Virus / immunology*
Nucleocapsid Proteins / immunology*
Recombinant Proteins / immunology
Swine

Substances

Antigens, Viral
Epitopes
Hepatitis B Antibodies
Hepatitis B Core Antigens
Nucleocapsid Proteins
Recombinant Proteins