Abstract
We studied changes in gene expression induced by the carbosilane dendrimer 2G-NN16 to evaluate their potential as a vehicle for gene therapy and as medication. Global gene expression profiles on CD8+ T lymphocytes reveal that ribosomal proteins are induced in the presence of 2G-NN16. IL17A and IL17F, the principal interleukins secreted by Tc17 cells, a subset of CD8+ T lymphocytes, were down-regulated when cultured in the presence of this dendrimer. Microarray results were confirmed by real time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). 2G-NN16 also showed a high potential for in vitro inhibition of Tc17 differentiation of CD8+ T lymphocytes in the presence of the Tc17 differentiation molecules IL6 and TGF-B1. These findings suggest that 2G-NN16 could facilitate drug delivery and may be used to treat inflammatory processes driven by Tc17 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / adverse effects
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / drug effects*
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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Cell Differentiation / drug effects*
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Cell Survival / drug effects
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Cells, Cultured
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Dendrimers / adverse effects
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Dendrimers / chemistry
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Dendrimers / pharmacology*
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Down-Regulation / drug effects*
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Gene Expression Profiling
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Gene Transfer Techniques / adverse effects
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Humans
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Interleukin-17 / genetics
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Interleukin-17 / metabolism
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Nanoparticles / adverse effects
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Nanoparticles / chemistry
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Oligonucleotide Array Sequence Analysis
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RNA, Messenger / metabolism
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Silanes / adverse effects
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Silanes / chemistry
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Silanes / pharmacology*
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Up-Regulation / drug effects
Substances
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2G-NN16
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Anti-Inflammatory Agents, Non-Steroidal
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Dendrimers
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IL17A protein, human
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IL17F protein, human
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Interleukin-17
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RNA, Messenger
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Ribosomal Proteins
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Silanes
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carbosilane