Antidepressant-like effects of the saponins extracted from Chaihu-jia-longgu-muli-tang in a rat unpredictable chronic mild stress model

Abstract

Chaihu-jia-longgu-muli-tang (CLM) has been used for treating depressive disorders for thousands of years in China. In the present study, we investigated the antidepressant-like effect of the saponins extracted from CLM (SCLM) in rats subjected to unpredictable chronic mild stress (UCMS). The ameliorative effect of SCLM on symptom of depression through behavior tests including: sucrose preference test, open-field test and forced-swimming test was investigated. In addition, high performance liquid chromatography with electrochemical detection (HPLC-ECD), immunohistochemical staining analysis and RT-PCR were applied to explore the mechanisms underlying the antidepressant-like effects of SCLM. It was observed that administration of SCLM (70, 140 mg/kg) reversed the depressive-like behaviors, restored the reduction in the levels of monoamine neurotransmitters and up-regulated the expression of brain-derived neurotrophic factor (BDNF) in UCMS-treated rats. These findings confirmed the antidepressant-like effects of SCLM in UCMS model of rats.