Abstract
A crucial issue in studies of morphogen gradients relates to their range: the distance over which they can act as direct regulators of cell signaling, gene expression and cell differentiation. To address this, we present a straightforward statistical framework that can be used in multiple developmental systems. We illustrate the developed approach by providing a point estimate and confidence interval for the spatial range of the graded distribution of nuclear Dorsal, a transcription factor that controls the dorsoventral pattern of the Drosophila embryo.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Animals
-
Biostatistics / methods*
-
Cleavage Stage, Ovum / metabolism
-
Computational Biology* / methods
-
Computational Biology* / statistics & numerical data
-
Computer Simulation
-
Drosophila / embryology*
-
Drosophila / genetics
-
Drosophila / metabolism*
-
Drosophila Proteins / analysis
-
Drosophila Proteins / genetics
-
Drosophila Proteins / metabolism*
-
Embryo, Nonmammalian / chemistry
-
Embryo, Nonmammalian / metabolism
-
Gene Expression Regulation, Developmental
-
Genes, Developmental* / physiology
-
Imaging, Three-Dimensional
-
In Situ Hybridization, Fluorescence
-
Morphogenesis / genetics*
-
Morphogenesis / physiology
-
Osmolar Concentration
-
Tissue Distribution / genetics