Neuropeptide FF receptor modulates potassium currents in a dorsal root ganglion cell line

Pharmacol Rep. 2011;63(4):1061-5. doi: 10.1016/s1734-1140(11)70623-5.

Abstract

This study investigated the presence of neuropeptide FF (NPFF) receptors on F-11 cells, a hybridoma derived from rat dorsal root ganglia (DRG) and mouse neuroblastoma. Binding experiments revealed a low density (4 fmol/mg) of high affinity (0.5 nM) [(3)H]-EYF binding sites in these cells. The whole-cell planar patch-clamp technique showed that dNPA, a selective NPFF(2) agonist, increased the voltage-dependent potassium outward currents (about 30 pA/pF) by 21%; this reversible effect on sustained delayed potassium currents is blocked by tetraethylammonium. The similar effects of NPFF and opioid agonists on K(+) currents in this cell line may explain their similar antinociceptive actions at the spinal level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesics, Opioid / pharmacology
  • Animals
  • Binding Sites
  • Cell Line
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / metabolism
  • Hybridomas*
  • Mice
  • Neuroblastoma / metabolism
  • Oligopeptides / pharmacology*
  • Patch-Clamp Techniques
  • Potassium Channels / drug effects
  • Potassium Channels / metabolism*
  • Rats
  • Receptors, Neuropeptide / drug effects
  • Receptors, Neuropeptide / metabolism*
  • Tetraethylammonium / pharmacology

Substances

  • Analgesics, Opioid
  • Oligopeptides
  • Potassium Channels
  • Receptors, Neuropeptide
  • neuropeptide FF receptor
  • Tetraethylammonium
  • phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide