Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR

J Clin Microbiol. 2011 Dec;49(12):4225-30. doi: 10.1128/JCM.00334-11. Epub 2011 Oct 12.

Abstract

We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.

Publication types

  • Evaluation Study

MeSH terms

  • Aged
  • Bacteriological Techniques / methods*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Osteoarthritis / microbiology*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Staphylococcus / classification*
  • Staphylococcus / genetics
  • Staphylococcus / isolation & purification*
  • Time Factors