Preparation of high purity biphenyl cyclooctene lignans from Schisandra extract by ion exchange resin catalytic transformation combined with macroporous resin separation

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3444-51. doi: 10.1016/j.jchromb.2011.09.022. Epub 2011 Sep 18.

Abstract

In this study, ester-bond biphenyl cyclooctene lignans were efficiently hydrolytically degraded into free biphenyl cyclooctene lignans by ion exchange resin transformation and simultaneous removal of impurities by macroporous resin. The OH-type strongly basic anion exchange resin 201×7 was the best one, and the dynamic hydrolysis efficiency was 146.7±5.0%. HPD5000 macroporous resin, which offered higher adsorption and desorption capacities and faster adsorption than other resins. The purity of free biphenyl cyclooctene lignans in the product increased from 5.14±0.24% to 79.67±0.0.67%. After dynamic catalytic transformation by 201×7 resin combined with purification of HPD5000 resin, the yield and the purity of free biphenyl cyclooctene lignans in the product were 132.1±4.7% and 80.91±3.53%, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Analysis of Variance
  • Chromatography, Ion Exchange / methods*
  • Cyclooctanes / isolation & purification*
  • Dioxoles / isolation & purification*
  • Ethanol
  • Fruit / chemistry
  • Hydrolysis
  • Ion Exchange Resins
  • Lignans / isolation & purification*
  • Plant Extracts / chemistry
  • Polycyclic Compounds / isolation & purification*
  • Schisandra / chemistry*
  • Spectrometry, Mass, Electrospray Ionization
  • Temperature

Substances

  • Cyclooctanes
  • Dioxoles
  • Ion Exchange Resins
  • Lignans
  • Plant Extracts
  • Polycyclic Compounds
  • Ethanol
  • schizandrin A
  • schizandrer A
  • schizandrin