Effect of AOT-assisted multi-walled carbon nanotubes on antibacterial activity

Abstract

The dispersing power of surfactant-modified multiwalled carbon nanotubes (MWCNTs) and their effect on the antibacterial activity were examined. The MWCNTs were modified using a dioctyl sodium sulfosuccinate (AOT) surfactant. UV-vis spectroscopy and transmission electron microscopy (TEM) were used to characterize the dispersion of MWCNTs in the aqueous phase. Fourier transform infrared spectroscopy confirmed the results of UV-vis spectroscopy and TEM, indicating that the AOT molecules had been adsorbed successfully onto the MWCNT surface. The highly dispersed AOT-modified MWCNTs showed strong antibacterial activity to Streptococcus mutans. The fluorescence images showed that the AOT-modified MWCNTs were capable of capturing bacteria and forming cell aggregates as well as killing them. The optical density growth curves and colony-forming units assays confirmed that the antibacterial activity of the AOT-modified MWCNTs was concentration-dependent and treatment time-dependent. This finding might be useful for applications of AOT-modified MWCNTs as an antibacterial agent to eliminate pathogens from a biocontaminated water phase.