Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells

J Cell Biochem. 2012 Feb;113(2):563-70. doi: 10.1002/jcb.23380.

Abstract

Retinoic acid (RA) is able to induce the differentiation of embryonic stem cells into neuronal lineages. The mechanism of this effect is unknown but it has been evidenced to be dependent on the formation of floating spheroids called embryoid bodies. Results presented here show that the inhibition of phosphoinositide 3-kinase signaling pre-determines mouse embryonic stem cells to RA induced neurogenesis in monolayer culture with no need of embryoid bodies formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / genetics
  • Antigens, Differentiation / metabolism
  • Cadherins / genetics
  • Cadherins / metabolism
  • Cell Culture Techniques
  • Cell Shape / drug effects
  • Cells, Cultured
  • Chromones / pharmacology*
  • Embryonic Stem Cells / drug effects
  • Embryonic Stem Cells / metabolism
  • Embryonic Stem Cells / physiology*
  • Gene Expression Regulation, Developmental / drug effects
  • Genes, Reporter
  • Keratin-8 / genetics
  • Keratin-8 / metabolism
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Mice
  • Morpholines / pharmacology*
  • Neural Cell Adhesion Molecules / genetics
  • Neural Cell Adhesion Molecules / metabolism
  • Neurogenesis / drug effects*
  • Phosphatidylinositol 3-Kinase / metabolism
  • Phosphoinositide-3 Kinase Inhibitors*
  • Signal Transduction / drug effects
  • Transcription, Genetic
  • Tretinoin / pharmacology*
  • Tubulin / genetics
  • Tubulin / metabolism

Substances

  • Antigens, Differentiation
  • Cadherins
  • Chromones
  • Keratin-8
  • Morpholines
  • Neural Cell Adhesion Molecules
  • Phosphoinositide-3 Kinase Inhibitors
  • Tubulin
  • beta3 tubulin, mouse
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Tretinoin
  • Luciferases
  • Phosphatidylinositol 3-Kinase