Abstract
The rabies Flury Low Egg Passage virus (LEP) has been widely used as a seed virus to generate inactive vaccine. Here, we established a reverse genetic system for LEP and generated a recombinant LEP virus (rLEP-G) that carries two identical G genes. This recombinant virus showed similar properties to those of LEP with respect to in vitro growth, neurotropism index, and virulence in mice. rLEP-G produced 4.3-fold more G protein than did LEP in BHK-21 cells. The inactivated vaccine generated from rLEP-G induced significantly higher virus neutralization titers in mice and dogs than those produced in response to LEP-derived vaccine. Our results suggest that rLEP-G is an improved seed virus candidate for inactivated rabies virus vaccine manufacture.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis*
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Antigens, Viral / chemistry
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Antigens, Viral / genetics
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Antigens, Viral / immunology*
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Blotting, Western
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Cell Line
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Cricetinae
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Dogs
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Female
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Glycoproteins / chemistry
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Glycoproteins / genetics
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Glycoproteins / immunology*
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Immunization*
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Injections, Intramuscular
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Mice
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Mice, Inbred BALB C
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Plasmids
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Polymerase Chain Reaction
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Rabies / immunology
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Rabies / prevention & control*
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Rabies / veterinary
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Rabies / virology
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Rabies Vaccines / administration & dosage*
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Rabies Vaccines / genetics
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Rabies Vaccines / immunology
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Rabies virus / chemistry
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Rabies virus / genetics
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Rabies virus / immunology*
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Rabies virus / pathogenicity
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Transduction, Genetic
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Vaccines, Inactivated / administration & dosage*
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Vaccines, Inactivated / genetics
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Vaccines, Inactivated / immunology
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Vaccines, Synthetic / administration & dosage*
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Viral Envelope Proteins / chemistry
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Virulence / genetics
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Virulence / immunology
Substances
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Antibodies, Viral
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Antigens, Viral
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Glycoproteins
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Rabies Vaccines
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Vaccines, Inactivated
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Vaccines, Synthetic
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Viral Envelope Proteins
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glycoprotein G, Rabies virus