Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts

Michal Afri; Miriam E Naqqash; Aryeh A Frimer

doi:10.1016/j.chemphyslip.2011.09.002

Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts

Chem Phys Lipids. 2011 Nov;164(8):759-65. doi: 10.1016/j.chemphyslip.2011.09.002. Epub 2011 Sep 16.

Authors

Michal Afri¹, Miriam E Naqqash, Aryeh A Frimer

Affiliation

¹ Department of Chemistry, Bar Ilan University, Ramat Gan, Israel. Michal.Afri@biu.ac.il

PMID: 21939642
DOI: 10.1016/j.chemphyslip.2011.09.002

Abstract

In previous work, we have shown the utility of the "NMR technique" in locating intercalants within the lipid bilayer. We describe herein the development of a more sensitive and complementary "fluorescence technique" for this purpose and its application to liposomes, bioliposomes and erythrocyte ghosts. This technique is based on the observation in selected compounds of an excellent correlation between the emission wavelength (λ(em)) and Dimroth-Reichardt E(T)(30) polarity parameter for the solvent in which the fluorescence emission spectrum was obtained.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Membrane*
Erythrocyte Membrane / chemistry
Fluorescence*
Ligands
Lipid Bilayers / chemistry
Liposomes / chemistry
Models, Biological
Solvents

Substances

Ligands
Lipid Bilayers
Liposomes
Solvents