[A comparison of the myogenic characteristics between stem cell antigen-(1+) and stem cell antigen-(1-) cells derived from skeletal muscle]

Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Aug;29(4):369-71, 374.
[Article in Chinese]

Abstract

Objective: To probe into the function of stem cell antigen-1 (Sca-1) in cell proliferation and differentiation.

Methods: Sca-(1+) and Sca-(1-) muscle derived cells were separated from C57BU6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca(-1), MyoD and Myogenin expression in cultured Sca(-1+) and Sca-(1-) muscle derived cells.

Results: The difference of the proliferative curve of Sca-(1+) and Sca-(1-) muscle derived cells cultured 3 days in vitro was not apparent, but Sca-(1-) muscle derived cells had a accelerated division rate in the follow days compared to the Sca-(1+) muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-(1+) than Sca-(1-) muscle derived cells.

Conclusion: Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.

MeSH terms

  • Animals
  • Antigens, Ly
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured*
  • Flow Cytometry
  • Membrane Proteins
  • Mice
  • Muscle, Skeletal
  • Myogenin*
  • Stem Cells

Substances

  • Antigens, Ly
  • Ly6a protein, mouse
  • Membrane Proteins
  • Myogenin