Abstract
Peptide nucleic acids containing 2-pyrimidinone (P) and 3-oxo-2,3-dihydropyridazine (E) heterocycles recognized C-G and U-A inversions in a polypurine tract of double helical RNA with high affinity and sequence selectivity at pH 6.25. E-modified PNA bound strongly to bacterial A-site RNA, while no binding was observed to the human A-site RNA.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Humans
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Inverted Repeat Sequences
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Nucleic Acid Conformation*
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Peptide Nucleic Acids / chemistry*
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Peptide Nucleic Acids / metabolism*
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Purines / metabolism*
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Pyrimidinones / metabolism*
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RNA / chemistry*
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RNA / genetics
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RNA / metabolism*
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Substrate Specificity
Substances
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Peptide Nucleic Acids
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Purines
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Pyrimidinones
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RNA