Triple helical recognition of pyrimidine inversions in polypurine tracts of RNA by nucleobase-modified PNA

Chem Commun (Camb). 2011 Oct 21;47(39):11125-7. doi: 10.1039/c1cc14706d. Epub 2011 Sep 12.

Abstract

Peptide nucleic acids containing 2-pyrimidinone (P) and 3-oxo-2,3-dihydropyridazine (E) heterocycles recognized C-G and U-A inversions in a polypurine tract of double helical RNA with high affinity and sequence selectivity at pH 6.25. E-modified PNA bound strongly to bacterial A-site RNA, while no binding was observed to the human A-site RNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Humans
  • Inverted Repeat Sequences
  • Nucleic Acid Conformation*
  • Peptide Nucleic Acids / chemistry*
  • Peptide Nucleic Acids / metabolism*
  • Purines / metabolism*
  • Pyrimidinones / metabolism*
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism*
  • Substrate Specificity

Substances

  • Peptide Nucleic Acids
  • Purines
  • Pyrimidinones
  • RNA