13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water

Chem Commun (Camb). 2011 Oct 14;47(38):10800-2. doi: 10.1039/c1cc13310a. Epub 2011 Sep 5.

Abstract

NMR-based analysis of glycans by directly observing hydroxyl protons has been difficult because of their inherently fast exchange with water. We observed hydroxyl proton exchanges in a LewisX-LewisX interaction by using deuterium isotope shifts on (13)C-NMR. This strategy is suitable for analyzing weak interactions by identifying involved protons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Isotopes / chemistry
  • Deuterium / chemistry
  • Hydrogen Bonding
  • Lewis X Antigen / analogs & derivatives
  • Magnetic Resonance Spectroscopy
  • Polysaccharides / chemistry
  • Protons*
  • Temperature
  • Trisaccharides / chemistry*
  • Water / chemistry*

Substances

  • Carbon Isotopes
  • Lewis X Antigen
  • Polysaccharides
  • Protons
  • Trisaccharides
  • galactosyl-(1,4)-fucopyranosyl-(1,3)-N-acetylglucosamine
  • Water
  • Deuterium