Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples

Ximei Wu; Jun Hu; Binghui Zhu; Lin Lu; Xiangdong Huang; Daiwen Pang

doi:10.1016/j.chroma.2011.08.045

Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples

J Chromatogr A. 2011 Oct 14;1218(41):7341-6. doi: 10.1016/j.chroma.2011.08.045. Epub 2011 Aug 19.

Authors

Ximei Wu¹, Jun Hu, Binghui Zhu, Lin Lu, Xiangdong Huang, Daiwen Pang

Affiliation

¹ College of Chemistry and Molecular Sciences, Wuhan University, Luojiashan, Wuhan, China. wxmdwb927@126.com

PMID: 21890142
DOI: 10.1016/j.chroma.2011.08.045

Abstract

A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5-50 μg/kg yielded mean recoveries from 67% to 90%, respectively. Finally, this oligosorbent was applied to the selective extraction of ochratoxin A from unfortified food samples.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Food Analysis / methods*
Magnetite Nanoparticles / chemistry*
Nanospheres / chemistry
Ochratoxins / analysis*
Reproducibility of Results
Solid Phase Extraction / instrumentation*

Substances

Aptamers, Nucleotide
Magnetite Nanoparticles
Ochratoxins
ochratoxin A