Abstract
A new labeling technique attaching a fluorescent pteridine derivative (3, 5) via a linker onto the 3'-OH group of 5'-O-dimethoxytritylthymidine (7) was developed to lead to the conjugates 8 and 11. After detritylation to give 9 and 12, the final conversion into the corresponding 5'-triphosphates (13, 14), which were isolated as sodium salts, was performed by known methods.
MeSH terms
-
Chemistry Techniques, Synthetic / methods*
-
Fluorescent Dyes / chemical synthesis*
-
Fluorescent Dyes / chemistry
-
Polyphosphates / chemical synthesis*
-
Polyphosphates / chemistry
-
Pteridines / chemical synthesis
-
Pteridines / chemistry
-
Thymidine / analogs & derivatives
-
Thymidine / chemical synthesis*
-
Thymidine / chemistry
Substances
-
Fluorescent Dyes
-
Polyphosphates
-
Pteridines
-
5'-O-dimethyltritylthymidine
-
triphosphoric acid
-
Thymidine