Inoculation of endophytic bacteria on host and non-host plants--effects on plant growth and Ni uptake

Abstract

Among a collection of Ni resistant endophytes isolated from the tissues of Alyssum serpyllifolium, four plant growth promoting endophytic bacteria (PGPE) were selected based on their ability to promote seedling growth in roll towel assay. Further, the PGPE screened showed the potential to produce plant growth promoting (PGP) substances and plant polymer hydrolyzing enzymes. These isolates were further screened for their PGP activity on A. serpyllifolium and Brassica juncea under Ni stress using a phytagar assay. None of the four isolates produced any disease symptoms in either plant. Further, strain A3R3 induced a maximum increase in biomass and Ni content of plants. Based on the PGP potential in phytagar assay, strain A3R3 was chosen for studying its PGP effect on A. serpyllifolium and B. juncea in Ni contaminated soil. Inoculation with A3R3 significantly increased the biomass (B. juncea) and Ni content (A. serpyllifolium) of plants grown in Ni contaminated soil. The strain also showed high level of colonization in tissue interior of both plants. By 16S rRNA gene sequencing analysis, A3R3 was identified as Pseudomonas sp. Successful colonization and subsequent PGP potentiality of Pseudomonas sp. A3R3 indicate that the inoculation with PGPE might have significant potential to improve heavy metal phytoremediation.