Abstract
The purpose of this study is to assess the potential of dendritic cells transfected with PD-L1 recombinant adenovirus induces CD8+ T cell suppression and kidney allograft tolerance. To prove it, DCs transfected with PD-L1 recombinant adenovirus (DC/Ad-PD-L1) were transferred into the MHC-mismatched rat kidney transplants. After kidney transplantation, the mixed lymphocyte reaction (MLR) assay and kidney function were analyzed. The results demonstrated that after administration of DC/Ad-PD-L1, the proliferation, cytokines secretion and activation marker expression of CD8+ T cells were suppressed. In addition, DC/Ad-PD-L1 could improve kidney function and survival of transplants. The findings suggested that DC/Ad-PD-L1 could induce CD8+ T cell tolerance and lead to kidney allograft tolerance, which provided a promising finding for clinical application.
Copyright © 2011 Elsevier Inc. All rights reserved.
MeSH terms
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Adenoviridae / genetics
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Animals
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B7-H1 Antigen / genetics
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B7-H1 Antigen / immunology*
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B7-H1 Antigen / metabolism
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Blotting, Western
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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Cell Proliferation
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Coculture Techniques
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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HEK293 Cells
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Humans
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Interferon-gamma / immunology
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Interferon-gamma / metabolism
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Interleukin-2 / immunology
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Interleukin-2 / metabolism
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Kidney Transplantation / methods
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Lymphocyte Culture Test, Mixed
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Male
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Proteinuria / immunology
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Proteinuria / urine
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Rats
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Rats, Inbred F344
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Rats, Inbred Lew
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology*
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Recombinant Fusion Proteins / metabolism
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Survival Analysis
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T-Lymphocytes / immunology*
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T-Lymphocytes / metabolism
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Time Factors
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Transfection
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Transplantation Tolerance / immunology*
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Transplantation, Homologous
Substances
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B7-H1 Antigen
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Interleukin-2
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Recombinant Fusion Proteins
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Interferon-gamma