Abstract
This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arterivirus Infections / diagnosis
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Arterivirus Infections / veterinary*
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Arterivirus Infections / virology
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Equartevirus / genetics
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Equartevirus / isolation & purification*
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Horse Diseases / diagnosis*
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Horse Diseases / virology
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Horses
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Immunomagnetic Separation / methods*
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RNA, Viral / genetics
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RNA, Viral / isolation & purification*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Semen / virology*
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Sensitivity and Specificity
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Veterinary Medicine / methods
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Virology / methods