The effects of the probiotic, Propionibacterium jensenii 702 (PJ 702), supplementation on egg productivity, egg shell thickness, fatty acid profile of eggs, and body weight in early layer hens were investigated. Twenty eight twenty-week-old starter pullets were evenly divided into a treatment and a control group for an eight week experiment. Each bird in the treatment group received 107 cfu PJ 702 daily in a total volume of 1 ml by oral administration. No adverse effect was observed due to administration of PJ 702, and successful gastrointestinal transit in the bird was demonstrated by recovery of PJ 702 from faeces of the treatment group. Layer production was significantly improved by the supplementation of PJ 702. Total egg weight in the treatment group was significantly higher than the control (P<0.001). Average egg weight for the treatment group was 55.26 g, 4.2% higher than the control which averaged 53.02 g. Moreover, the fatty acid profile was significantly altered by the supplementation of PJ 702. Myristic acid (P<0.001), palmitoleic acid (P=0.001) and all-cis-11,14-eicosadienoic acid (P=0.02) were significantly lower in the treatment group compared to the control group. No difference in egg shell thickness was observed between the treatment and control group (P=0.23). In conclusion, the application of novel probiotic PJ 702 in the early layer hen is safe and effective to promote production and the quality of products in layer husbandry.