Objective: To study the sterol constituents of Bugula neritina from the South China Sea.
Methods: The alcohol extract of Bugula neritina was purified by silica gel column and Sephadex LH-20 column chromatography, and structures of the isolated compounds were identified by spectroscopic analysis and comparison with those of literatures.
Results: Ten sterols were isolated and identified from the petroleum ether fraction of alcohol extract of B. neritina L.:Cholest-4-en-3-one(I); cholesterol(II);3beta,5alpha,9alpha-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(III); 24-methyl-5alpha-cholesta-7,22-diene-3beta,5,6beta-triol(IV); 3beta-Hydroxy-7-methoxy-Cholesta-5-en(V);5alpha, 8alpha-epidioxy-(22E,24R)-ergosta-6, 22-dien-3beta-ol(VI); 3beta-hydroxycholest-5-en-7-one (VII); and 6beta-hydroxy-cholest-4-en-3-one(VIII); Cholesta-5-ene-3beta,7beta-diol(IX); Cholesta-5,22(E)-dien-3beta,7alpha-diol(X).
Conclusion: Compounds IV-X were isolated from Bugula neritina for the first time.