Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase

Chao Gao; Xiaoman Xu; Xifeng Zhang; Bin Che; Cuiqing Ma; Jianhua Qiu; Fei Tao; Ping Xu

doi:10.1128/AEM.05601-11

Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase

Appl Environ Microbiol. 2011 Oct;77(19):7080-3. doi: 10.1128/AEM.05601-11. Epub 2011 Aug 5.

Authors

Chao Gao¹, Xiaoman Xu, Xifeng Zhang, Bin Che, Cuiqing Ma, Jianhua Qiu, Fei Tao, Ping Xu

Affiliation

¹ State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

Abstract

Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosamine / metabolism*
Aldehyde-Lyases / genetics
Aldehyde-Lyases / metabolism*
Bacillus subtilis / genetics
Bacillus subtilis / metabolism*
Biosynthetic Pathways / genetics
Biotechnology / methods
Gene Expression*
N-Acetylneuraminic Acid / biosynthesis*
Organisms, Genetically Modified
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Spores, Bacterial / genetics
Spores, Bacterial / metabolism*

Substances

Recombinant Proteins
Aldehyde-Lyases
neuraminic acid aldolase
N-Acetylneuraminic Acid
Acetylglucosamine