A minD mutant of enterohemorrhagic E. coli O157:H7 has reduced adherence to human epithelial cells

Microb Pathog. 2011 Nov;51(5):378-83. doi: 10.1016/j.micpath.2011.07.003. Epub 2011 Jul 20.

Abstract

Adherence to epithelial cells is a prerequisite for intestinal colonization by the bacterial pathogen, enterohemorrhagic Escherichia coli (EHEC). The deletion of minD, a cell division gene, in EHEC caused reduced adherence to human epithelioid cervical carcinoma (HeLa) and human colonic adenocarcinoma (Caco-2) cells as compared to wild-type. The minD mutant formed minicells and filaments owing to aberrant cytokinesis. Moreover, its ability to form microcolonies as typically seen in the co-cultures of wild-type with Caco-2 cells, was abolished. In conclusion, the present study highlights the importance of minD in regards to EHEC adherence to human epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / metabolism*
  • Bacterial Adhesion*
  • Caco-2 Cells
  • Epithelial Cells / microbiology*
  • Escherichia coli Infections / microbiology*
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / physiology*
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism*
  • HeLa Cells
  • Humans
  • Mutation*

Substances

  • Escherichia coli Proteins
  • Adenosine Triphosphatases
  • MinD protein, E coli