Abstract
A heterologous gene expression system was created in a domestic Aspergillus awamori Co-6804 strain, which is a producer of the glucoamylase gene. Vector pGa was prepared using promoter and terminator areas of the glucoamylase gene, and A. niger phytase, Trichoderma reesei endoglucanase, and Penicillium canescens xylanase genes were then cloned into pGa vector. Separation of enzyme samples using FPLC showed the amount of the recombinant proteins to be within the 0.6-14% range of total protein.
MeSH terms
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6-Phytase / genetics
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6-Phytase / metabolism
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Aspergillus / enzymology
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Aspergillus / genetics*
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Base Sequence
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Biotechnology
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Cellulase / genetics
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Cellulase / metabolism
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Cloning, Molecular
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Gene Expression Regulation, Fungal*
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Genetic Engineering
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Genetic Vectors / chemistry*
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Genetic Vectors / isolation & purification*
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Glucan 1,4-alpha-Glucosidase / genetics
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Glucan 1,4-alpha-Glucosidase / metabolism
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Molecular Sequence Data
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Penicillium / chemistry
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Penicillium / enzymology
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Promoter Regions, Genetic
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / genetics
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Terminator Regions, Genetic
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Trichoderma / chemistry
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Trichoderma / enzymology
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Xylan Endo-1,3-beta-Xylosidase / genetics
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Xylan Endo-1,3-beta-Xylosidase / metabolism
Substances
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Recombinant Proteins
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6-Phytase
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Glucan 1,4-alpha-Glucosidase
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Xylan Endo-1,3-beta-Xylosidase
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Cellulase