Theranostic systems assembled in situ on demand by host-guest chemistry

Biomaterials. 2011 Oct;32(30):7687-94. doi: 10.1016/j.biomaterials.2011.06.060. Epub 2011 Jul 23.

Abstract

Theranostic systems have been explored extensively for a diagnostic therapy in the forms of polymer conjugates, implantable devices, and inorganic nanoparticles. In this work, we report theranostic systems in situ assembled by host-guest chemistry responding to a request. As a model theranostic system on demand, cucurbit[6]uril-conjugated hyaluronate (CB[6]-HA) was synthesized and decorated with FITC-spermidine (spmd) and/or formyl peptide receptor like 1 (FPRL1) specific peptide-spmd by simple mixing in aqueous solution. The resulting (FITC-spmd and/or peptide-spmd)@CB[6]-HA was successfully applied to the bioimaging of its target-specific delivery to B16F1 cells with HA receptors and its therapeutic signal transduction with elevated Ca(2+) and phosphor-extracellular signal-regulated kinase (pERK) levels in FPRL1-expressing human breast adenocarcinoma (FPRL1/MCF-7) cells. Finally, we could confirm in vitro and in vivo stability of the highly specific host-guest interaction. The on-demand theranostic platform technology using host-guest chemistry can be exploited for various bioimaging, biosensing, drug delivery, and tissue engineering applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / diagnosis
  • Adenocarcinoma / therapy
  • Animals
  • Breast Neoplasms / diagnosis
  • Breast Neoplasms / therapy
  • Bridged-Ring Compounds* / chemistry
  • Bridged-Ring Compounds* / therapeutic use
  • Cell Line, Tumor
  • Female
  • Fluorescein-5-isothiocyanate* / chemistry
  • Fluorescein-5-isothiocyanate* / therapeutic use
  • Humans
  • Hyaluronic Acid* / chemistry
  • Hyaluronic Acid* / therapeutic use
  • Imidazoles* / chemistry
  • Imidazoles* / therapeutic use
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Formyl Peptide / analysis*
  • Receptors, Formyl Peptide / chemistry
  • Receptors, Formyl Peptide / therapeutic use
  • Receptors, Lipoxin / analysis*
  • Receptors, Lipoxin / chemistry
  • Receptors, Lipoxin / therapeutic use
  • Spermidine* / chemistry
  • Spermidine* / therapeutic use

Substances

  • Bridged-Ring Compounds
  • FPR2 protein, human
  • Imidazoles
  • Receptors, Formyl Peptide
  • Receptors, Lipoxin
  • cucurbit(6)uril
  • Hyaluronic Acid
  • Fluorescein-5-isothiocyanate
  • Spermidine