Evaluation of the colonization capabilities of Salmonella Enteritidis in quails using an RT-PCR approach

Guang-Zhi He; Yong Feng; Shu-Xuan Deng; Li-Fang He; Chuan-Wei An; Wei-Yi Tian

doi:10.1016/j.rvsc.2011.06.022

Evaluation of the colonization capabilities of Salmonella Enteritidis in quails using an RT-PCR approach

Res Vet Sci. 2012 Aug;93(1):28-30. doi: 10.1016/j.rvsc.2011.06.022. Epub 2011 Jul 20.

Authors

Guang-Zhi He¹, Yong Feng, Shu-Xuan Deng, Li-Fang He, Chuan-Wei An, Wei-Yi Tian

Affiliation

¹ Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China.

PMID: 21764092
DOI: 10.1016/j.rvsc.2011.06.022

Abstract

We used a real-time PCR assay and indirect fluorescent antibody (IFA) assay to detect genomic DNA of Salmonella Enteritidis in the internal organs of quails after an oral challenge. The results showed that S. Enteritidis was detected in all the samples at different time points. This study will assist a future understanding of the pathogenesis of S. Enteritidis.

MeSH terms

Animals
Bird Diseases / genetics
Bird Diseases / microbiology*
Fluorescent Antibody Technique, Indirect / veterinary
Quail / microbiology*
Real-Time Polymerase Chain Reaction / veterinary*
Salmonella Infections, Animal / genetics
Salmonella Infections, Animal / microbiology*
Salmonella Infections, Animal / pathology
Salmonella enteritidis / genetics
Salmonella enteritidis / pathogenicity*