Multiplexed binding determination of seven glycoconjugates for Pseudomonas aeruginosa lectin I (PA-IL) using a DNA-based carbohydrate microarray

Chem Commun (Camb). 2011 Aug 21;47(31):8826-8. doi: 10.1039/c1cc12428e. Epub 2011 Jul 11.

Abstract

The binding of seven multivalent glycoconjugates displaying linear or antenna-like structures and different electronic environments were evaluated towards PA-IL on a DNA-based carbohydrate microarray. The affinity can be modulated by the charge and the topology of the galactosylated derivatives.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrates / chemistry*
  • Click Chemistry
  • DNA / chemistry
  • DNA / metabolism*
  • Glycoconjugates / chemistry
  • Glycoconjugates / metabolism*
  • Lectins / metabolism*
  • Microarray Analysis
  • Protein Binding
  • Pseudomonas aeruginosa / metabolism*
  • Spectrometry, Fluorescence

Substances

  • Carbohydrates
  • Glycoconjugates
  • Lectins
  • DNA