Abstract
Seven new cembranoids, sarcocrassocolides F-L (1-7), have been isolated from a soft coral Sarcophyton crassocaule. Their structures were determined by extensive spectroscopic analysis. Most new compounds exhibited significant cytotoxic activity against a limited panel of cancer cell lines, and the structure-activity relationship was studied. Compounds 1-7 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Compound 4 was also found to effectively reduce the level of COX-2 protein.
Keywords:
Sarcophyton crassocaule; anti-inflammatory activity; cytotoxic activity; soft coral.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anthozoa / chemistry*
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology
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Cell Line, Tumor
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Cyclooxygenase 2 / metabolism
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Diterpenes / chemistry*
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Diterpenes / isolation & purification
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Diterpenes / pharmacology*
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Drug Screening Assays, Antitumor / methods
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Humans
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nitric Oxide Synthase Type II / metabolism
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Structure-Activity Relationship
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Taiwan
Substances
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Anti-Inflammatory Agents
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Diterpenes
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Lipopolysaccharides
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cembrane
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2