[Experience in using the LABType SSO reagent kits in the practice of a HLA typing laboratory]

Klin Lab Diagn. 2011 Apr:(4):45-7.
[Article in Russian]

Abstract

HLA was typed from HLA-A, HLA-B, HLA-DRB1 loci (to the second place) in 443 patients, by applying the LABType SSO reagent kits (One Lambda, USA). The findings were analyzed using the software Arlequin version 3.1. There were no deviations from the Hardy-Weinberg law. Overall, the authors identified 16, 27, and 13 allelic variants ofHLA-A, HLA-B, and HLA-DRB1 loci, respectively. There were also 4 most common haplotypes of HLA-A-B-DRBII: HLA-A *03-B*35-DRB1 *01 (4.29%), HLA-A *01-B*08-DRBl *03 (3.5%), HLA-A*03-B*07-DRBI *15 (3.37%), and HLA-A *02-B*07-DRBI *15 (2.93%).

Publication types

  • English Abstract

MeSH terms

  • Blood Donors
  • Gene Frequency
  • Genetics, Population
  • HLA Antigens / genetics
  • HLA Antigens / metabolism*
  • Haplotypes
  • Hematopoietic Stem Cell Transplantation
  • Histocompatibility Testing / methods
  • Histocompatibility Testing / standards*
  • Humans
  • Polymerase Chain Reaction
  • Population Groups*
  • Reagent Kits, Diagnostic / standards
  • Software

Substances

  • HLA Antigens
  • Reagent Kits, Diagnostic