Abstract
Facile, two-step synthesis and kinetic characterization of new chemical probes for selective labeling of sulfenic acid (-SOH) in proteins are presented. The synthesis route relies on the simple and highly efficient Michael addition of thiol containing tags or linkers to 4-cyclopentene-1,3-dione, the unsaturated derivative of 1,3-cyclopentanedione.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cyclopentanes / chemical synthesis*
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Cyclopentanes / chemistry
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Mice
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NIH 3T3 Cells
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Proteins / chemistry*
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Proteins / isolation & purification
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Staining and Labeling / methods
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Sulfenic Acids / chemistry*
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Sulfenic Acids / isolation & purification
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Sulfhydryl Compounds / chemical synthesis
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Sulfhydryl Compounds / chemistry
Substances
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Cyclopentanes
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Proteins
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Sulfenic Acids
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Sulfhydryl Compounds
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4-cyclopentene-1,3-dione