Sugar modified oligonucleotides. III (1). Synthesis, nuclease resistance and base pairing properties of alpha- and beta-L-octathymidylates

Biochem Biophys Res Commun. 1990 Oct 30;172(2):537-43. doi: 10.1016/0006-291x(90)90706-s.

Abstract

Two unnatural L-oligodeoxyribonucleotides, namely alpha- and beta-L-dT8pO(CH2)3OH, have been synthesized. These oligomers are resistant towards nuclease degradation. They do not show any UV detectable base pairing with beta-D-dA8 and poly rA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Cattle
  • Indicators and Reagents
  • Isomerism
  • Magnetic Resonance Spectroscopy
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism
  • Phosphoric Diester Hydrolases / metabolism
  • Single-Strand Specific DNA and RNA Endonucleases / metabolism
  • Spleen / enzymology

Substances

  • Indicators and Reagents
  • Oligodeoxyribonucleotides
  • oligo (dT)
  • Single-Strand Specific DNA and RNA Endonucleases
  • Phosphoric Diester Hydrolases