[Oligo(2'-O-methylribonucleotides) with the insertions of 2'-bispyrenylmethylphosphorodiamidate nucleoside derivatives as perspective fluorescent probes for RNA detection]

Abstract

A series of novel fluorescent conjugates of oligo(2'-O-methylribonucleotides) containing the insertions of 2'bispyrenylmethylphosphorodiamidate derivatives of ribonucleosides (Urd, Cyd) were synthesized and their fluorescent properties were investigated. The possibility of detection of RNA transcript of 5'-terminal fragment of mRNA of multidrug resistance gene mdr1 (1-678) in solution using synthesized multipyrene probes was demonstrated.