CD47 regulates the TGF-β signaling pathway in osteoblasts and is distributed in Meckel's cartilage

J Oral Sci. 2011 Jun;53(2):169-75. doi: 10.2334/josnusd.53.169.

Abstract

Previously, CD47 gene expression has been shown to increase during mandible development using a micro array technique. To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone metabolism increased dose-dependently with CD47 silencing. Furthermore, we determined the distribution of CD47 in mouse embryonic E13 and E15 in vivo. The CD47-positive cells were localized in Meckel's cartilage and antenatal mandibular bone. These results suggest that TGF-β1 signaling and mandible development might be regulated by CD47.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Blotting, Western
  • CD47 Antigen / analysis
  • CD47 Antigen / genetics
  • CD47 Antigen / physiology*
  • Cartilage / embryology*
  • Cell Culture Techniques
  • Electrophoresis, Polyacrylamide Gel
  • Gene Silencing / physiology
  • Gestational Age
  • Immunohistochemistry
  • Mandible / embryology*
  • Mandible / metabolism
  • Mesoderm / physiology*
  • Mice
  • Osteoblasts / physiology*
  • Polymerase Chain Reaction
  • RNA, Small Interfering / genetics
  • Signal Transduction / genetics
  • Signal Transduction / physiology*
  • Smad2 Protein / analysis
  • Smad2 Protein / genetics
  • Transcription Factors / genetics
  • Transforming Growth Factor beta1 / analysis
  • Transforming Growth Factor beta1 / genetics
  • Transforming Growth Factor beta1 / physiology*

Substances

  • CD47 Antigen
  • Cd47 protein, mouse
  • RNA, Small Interfering
  • Smad2 Protein
  • Smad2 protein, mouse
  • Transcription Factors
  • Transforming Growth Factor beta1