Objective: To develop an UPLC method of determining the characteristic chromatographic profiles of Paeoniae Radix Alba for quality control.
Method: The UPLC characteristic chromatographic profiles of fifteen batches of Paeoniae Radix Alba were determined on an HSS T3 Column (2.1 mm x 100 mm, 1.8 microm) eluted with the mobile phases of water containing 0.05% phosphoric acid and acetonitrile in gradient mode. The detection wavelength was set at 230 nm.
Result: The common mode of the UPLC characteristic chromatographic profile was set up. There were 15 common peaks, five of which were identified, and the similar degrees of the fifteen samples to the common mode were between 0.891 and 0.996.
Conclusion: The method was time-saving and can be used for the quality control of Paeoniae Radix Alba.