The ribosomal RNA is a useful marker to visualize rhizobia interacting with legume plants

Abstract

Symbiosis between rhizobia and leguminous plants leads to the formation of nitrogen-fixing root nodules. In the present article, we recommend the use of the ribosomal RNA (rRNA) isolated from legume nodules in an experimental class with the purpose of introducing students to the structure of eukaryotic and prokaryotic ribosomes and of demonstrating rhizobia colonization and nodule occupancy. We consider that this activity might help students previously trained in molecular biology handling to experience a richer way of learning some concepts and methodology in molecular plant-microbe interactions.