Branched RNA nanostructures for RNA interference

Yuko Nakashima; Hiroshi Abe; Naoko Abe; Kyoko Aikawa; Yoshihiro Ito

doi:10.1039/c1cc11780g

Branched RNA nanostructures for RNA interference

Chem Commun (Camb). 2011 Aug 7;47(29):8367-9. doi: 10.1039/c1cc11780g. Epub 2011 Jun 21.

Authors

Yuko Nakashima¹, Hiroshi Abe, Naoko Abe, Kyoko Aikawa, Yoshihiro Ito

Affiliation

¹ Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198, Japan.

PMID: 21691656
DOI: 10.1039/c1cc11780g

Abstract

Branched RNAs with three- or four-way junctions were designed by assembling single-stranded RNA for RNA interference. Human Dicer transformed branched RNAs into about 20 base pairs of double-stranded RNA, which is a standard siRNA species. Our tetramer design provides a potent silencing effect over a period of 5 days.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DEAD-box RNA Helicases / antagonists & inhibitors
DEAD-box RNA Helicases / genetics
DEAD-box RNA Helicases / metabolism
HeLa Cells
Humans
Nanostructures / chemistry*
RNA Interference*
RNA, Double-Stranded / chemistry*
RNA, Double-Stranded / metabolism
RNA, Small Interfering / metabolism
Ribonuclease III / antagonists & inhibitors
Ribonuclease III / genetics
Ribonuclease III / metabolism

Substances

RNA, Double-Stranded
RNA, Small Interfering
DICER1 protein, human
Ribonuclease III
DEAD-box RNA Helicases