Cellular resolution optical coherence microscopy with high acquisition speed for in-vivo human skin volumetric imaging

Opt Lett. 2011 Jun 15;36(12):2221-3. doi: 10.1364/OL.36.002221.

Abstract

In this Letter, we report for the first time (to our knowledge) in-vivo volumetric optical coherence microscopy images of skin epidermal cells. We achieved micrometer-class resolution, 2 μm laterally and axially, with an acquisition speed of 23 K A-scans/s and over 90 dB sensitivity to a depth of 1 mm by employing a custom, liquid-lens-based, dynamic-focusing objective, a broadband light source, and a custom, astigmatism-corrected Czerny-Turner spectrometer with a high-speed complementary metal-oxide-semiconductor camera.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Epidermal Cells
  • Humans
  • Microscopy / methods*
  • Skin / cytology*
  • Time Factors
  • Tomography, Optical Coherence / methods*