In this chapter, we describe standardized protocols for the self-assembly of supported lipid bilayers (SLBs) from liposomes with lipid compositions mimicking eukaryote and prokaryote cell membranes. Such SLBs can also contain lipids with polymeric and glycosylated headgroups. Furthermore, we present protocols on how to manipulate the adsorption and desorption of membranes on indium tin oxide (ITO) electrodes, which allows for the creation of patterned and in situ regenerated SLB arrays that can be used to study electrochemically mediated membrane processes in a microarray format.