Detecting weak protein-protein interactions by modified far-western blotting

Yuya Sato; Masafumi Kameya; Hiroyuki Arai; Masaharu Ishii; Yasuo Igarashi

doi:10.1016/j.jbiosc.2011.05.011

Detecting weak protein-protein interactions by modified far-western blotting

J Biosci Bioeng. 2011 Sep;112(3):304-7. doi: 10.1016/j.jbiosc.2011.05.011. Epub 2011 Jun 12.

Authors

Yuya Sato¹, Masafumi Kameya, Hiroyuki Arai, Masaharu Ishii, Yasuo Igarashi

Affiliation

¹ Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.

PMID: 21669550
DOI: 10.1016/j.jbiosc.2011.05.011

Abstract

We developed a cross-linking far-western blotting (WB) technique to detect weak protein-protein interactions. In addition, by comparison of the banding patterns resulting from the conventional and cross-linking methods, weak interactions can be identified by monitoring the enhancement of band intensities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Far-Western / methods*
Haemophilus / metabolism
Humans
Protein Interaction Maps
Proteins / metabolism*

Substances

Proteins