Measurement of rotavirus-neutralizing coproantibody in children by fluorescent focus reduction assay

J Clin Microbiol. 1990 Jul;28(7):1652-4. doi: 10.1128/jcm.28.7.1652-1654.1990.

Abstract

A fluorescent focus reduction assay suitable for the measurement of rotavirus-neutralizing antibodies in the feces of children was developed. Of 408 stools tested, 7% showed false-positive neutralization, and the number of rotavirus serotypes neutralized by a fecal extract was proportional to the levels of antirotaviral immunoglobulin A in the extract.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / analysis*
  • Child
  • Evaluation Studies as Topic
  • Feces / microbiology
  • Fluorescent Antibody Technique*
  • Gastroenteritis / immunology
  • Humans
  • Neutralization Tests
  • Rotavirus / immunology*
  • Rotavirus Infections / immunology

Substances

  • Antibodies, Viral