Effect of cyclosporine pretreatment on mitochondrial function in vitrified bovine mature oocytes

Fertil Steril. 2011 Jun 30;95(8):2786-8. doi: 10.1016/j.fertnstert.2011.04.089. Epub 2011 Jun 8.

Abstract

Vitrification had a significantly negative impact on the mitochondrial function of bovine oocytes. However, 40 μg/mL cyclosporine pretreatment before vitrification contributed greatly to maintaining mitochondrial membrane potential and adenosine triphosphate content, decreasing reactive oxygen species level, and thereby increasing the developmental ability of vitrified oocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / analogs & derivatives
  • Adenine / pharmacology
  • Adenosine Triphosphate / metabolism
  • Animals
  • Blastocyst / drug effects
  • Calcimycin / pharmacology
  • Cattle
  • Cryopreservation*
  • Cyclosporine / pharmacology*
  • Embryo Culture Techniques
  • Female
  • Ionophores / pharmacology
  • Membrane Potential, Mitochondrial / drug effects
  • Mitochondria / drug effects*
  • Mitochondria / metabolism
  • Oocytes / drug effects*
  • Oocytes / metabolism
  • Parthenogenesis / drug effects
  • Reactive Oxygen Species / metabolism
  • Time Factors
  • Vitrification*

Substances

  • Ionophores
  • Reactive Oxygen Species
  • Calcimycin
  • N(6),N(6)-dimethyladenine
  • Cyclosporine
  • Adenosine Triphosphate
  • Adenine