Determination of the Rh factor: A practical illustrating the use of the polymerase chain reaction

Santiago Imperial; Albert Boronat

doi:10.1002/bmb.2005.494033010425

Determination of the Rh factor: A practical illustrating the use of the polymerase chain reaction

Biochem Mol Biol Educ. 2005 Jan;33(1):50-3. doi: 10.1002/bmb.2005.494033010425.

Authors

Santiago Imperial¹, Albert Boronat

Affiliation

¹ Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Avda Diagonal 645, 08028-Barcelona, Spain. imperial@bq.ub.es.

PMID: 21638540
DOI: 10.1002/bmb.2005.494033010425

Abstract

A practical experiment on the PCR is described that has been used over several years as part of an undergraduate biochemistry and molecular biology course for chemistry students. In the first experimental session, students prepare their own DNA samples from epithelial cells of the mouth and use them as templates in the PCR. In the second session, they analyze the amplified DNA by electrophoresis and determine their Rh factor.