Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing

J Infect Dev Ctries. 2011 May 28;5(5):348-52. doi: 10.3855/jidc.1197.

Abstract

Introduction: We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.

Methodology: This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing.

Results: From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing.

Conclusions: The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Substitution / genetics*
  • Antiviral Agents / pharmacology
  • Disease Outbreaks
  • Drug Resistance, Viral*
  • Humans
  • Influenza A Virus, H1N1 Subtype / drug effects*
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / isolation & purification*
  • Influenza, Human / virology*
  • Male
  • Molecular Diagnostic Techniques / methods*
  • Mutation, Missense
  • Oseltamivir / pharmacology
  • Sequence Analysis, DNA / methods
  • Thailand
  • Virology / methods*

Substances

  • Antiviral Agents
  • Oseltamivir