Abstract
Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 10(9) organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 10(9) organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Bacterial / biosynthesis*
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Adhesins, Bacterial / immunology
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Adolescent
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Adult
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Antibodies, Bacterial / blood
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Bacterial Toxins / immunology
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Diarrhea / microbiology
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Diarrhea / pathology
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Enterotoxigenic Escherichia coli / growth & development
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Enterotoxigenic Escherichia coli / pathogenicity*
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Enterotoxins / immunology
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Escherichia coli Infections / microbiology*
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Escherichia coli Infections / pathology*
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Escherichia coli Proteins / biosynthesis*
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Escherichia coli Proteins / immunology
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Female
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Human Experimentation
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Humans
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Male
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Middle Aged
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Virulence Factors / biosynthesis*
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Virulence Factors / immunology
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Young Adult
Substances
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Adhesins, Bacterial
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Antibodies, Bacterial
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Bacterial Toxins
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Enterotoxins
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Escherichia coli Proteins
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Virulence Factors
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heat-labile enterotoxin, E coli